Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
-
Rauch Tibor Attila
senior research fellow,
Department of Medical Biology and Central Electron Microscope Laboratory -
Number of hours/semester
lectures: 16 hours
practices: 8 hours
seminars: 0 hours
total of: 24 hours
Subject data
- Code of subject: OTF-GBJ-T
- 2 kredit
- Biotechnology BSc
- Optional modul
- autumn
OTV-BIC1-T finished , OTV-SEBI-T finished
Course headcount limitations
min. 5 – max. 15
Available as Campus course for . Campus-karok: ÁOK ETK GYTK TTK
Topic
Genetic engineering techniques offer unprecedented therapeutic possibilities that will certainly play a central role in future medicine. Today's oncotherapy and rheumatology toolkits already rely to a large extent on genetically engineered protein and peptide-based drugs. During the COVID-19 epidemic, recombinant viral and RNA vaccines proved their efficacy. In the light of these facts, future scientists and clinicians will benefit from this course, which will provide them a theoretical background of genetic engineering and practical insights into basic recombinant DNA, RNA and protein expression technologies. Current legal and medical positions on the subject will also be discussed.
Lectures
- 1. The subject and history of genetic engineering - Rauch Tibor Attila
- 2. Ethical issues in genetic engineering - Rauch Tibor Attila
- 3. DNA, RNA and protein extraction from medical samples: past and present - Rauch Tibor Attila
- 4. Enzymes used in genetic engineering - Rauch Tibor Attila
- 5. Basics of molecular cloning - Rauch Tibor Attila
- 6. New genetic engineering technologies - Rauch Tibor Attila
- 7. Protein overproduction in prokaryotic and eukaryotic systems - Rauch Tibor Attila
- 8. Peptide- and protein-based therapies - Rauch Tibor Attila
- 9. Classical approach of antibody production - Rauch Tibor Attila
- 10. Production of antibodies with new technologies - Rauch Tibor Attila
- 11. Bispecific antibodies - Rauch Tibor Attila
- 12. CAR-T technology - Rauch Tibor Attila
- 13. Classical vaccine production - Rauch Tibor Attila
- 14. Recombinant viral vaccines - Rauch Tibor Attila
- 15. Recombinant RNA vaccines - Rauch Tibor Attila
- 16. Recombinant RNA- and DNA-based oncotherapy - Rauch Tibor Attila
Practices
- 1. RNA and DNA isolation from blood, PCR primer design - Rauch Tibor Attila
- 2. cDNA synthesis, PCR amplification - Rauch Tibor Attila
- 3. Cloning into protein expression vector - Rauch Tibor Attila
- 4. Recombinant protein overproduction and purification - Rauch Tibor Attila
- 5. Biochemical analysis of purified protein - Rauch Tibor Attila
- 6. Physiological testing of purified protein - Rauch Tibor Attila
- 7. Oral presentation of a chosen topic - Rauch Tibor Attila
- 8. Oral presentation of a chosen topic - Rauch Tibor Attila
Seminars
Reading material
Obligatory literature
Literature developed by the Department
PowerPoint slides of the course
Notes
Recommended literature
Conditions for acceptance of the semester
Test at the end of the semester
Mid-term exams
N/A
Making up for missed classes
N/A
Exam topics/questions
1 The subject and history of genetic engineering
2 Ethical issues in genetic engineering
3 DNA, RNA and protein extraction from medical samples: past and present
4 Enzymes used in genetic engineering
5 Basics of molecular cloning
6 New genetic engineering technologies
7 Peptide- and protein-based therapies
8 Protein overproduction in prokaryotic and eukaryotic systems
9 Classical approach of antibody production
10 Production of antibodies with new technologies
11 Bispecific antibodies
12 CAR-T technology
13 Classical vaccine production
14 Recombinant viral vaccines
15 Recombinant RNA vaccines
16 Recombinant RNA- and DNA-based oncotherapy
Examiners
Instructor / tutor of practices and seminars
- Rauch Tibor Attila