Medicine in the Future: Genetic Engineering and Biotechnology (Recombinant DNA/RNA/Protein Technologies)

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2024-2025

Thematik

Genetic engineering techniques offer unprecedented therapeutic possibilities that will certainly play a central role in future medicine. Today's oncotherapy and rheumatology toolkits already rely to a large extent on genetically engineered protein and peptide-based drugs. During the COVID-19 epidemic, recombinant viral and RNA vaccines proved their efficacy. In the light of these facts, future scientists and clinicians will benefit from this course, which will provide them a theoretical background of genetic engineering and practical insights into basic recombinant DNA, RNA and protein expression technologies. Current legal and medical positions on the subject will also be discussed.

Vorlesungen

• 1. The subject and history of genetic engineering - Rauch Tibor Attila
• 2. Ethical issues in genetic engineering - Rauch Tibor Attila
• 3. DNA, RNA and protein extraction from medical samples: past and present - Rauch Tibor Attila
• 4. Enzymes used in genetic engineering - Rauch Tibor Attila
• 5. Basics of molecular cloning - Rauch Tibor Attila
• 6. New genetic engineering technologies - Rauch Tibor Attila
• 7. Protein overproduction in prokaryotic and eukaryotic systems - Rauch Tibor Attila
• 8. Peptide- and protein-based therapies - Rauch Tibor Attila
• 9. Classical approach of antibody production - Rauch Tibor Attila
• 10. Production of antibodies with new technologies - Rauch Tibor Attila
• 11. Bispecific antibodies - Rauch Tibor Attila
• 12. CAR-T technology - Rauch Tibor Attila
• 13. Classical vaccine production - Rauch Tibor Attila
• 14. Recombinant viral vaccines - Rauch Tibor Attila
• 15. Recombinant RNA vaccines - Rauch Tibor Attila
• 16. Recombinant RNA- and DNA-based oncotherapy - Rauch Tibor Attila

Praktika

• 1. RNA and DNA isolation from blood, PCR primer design - Rauch Tibor Attila
• 2. cDNA synthesis, PCR amplification - Rauch Tibor Attila
• 3. Cloning into protein expression vector - Rauch Tibor Attila
• 4. Recombinant protein overproduction and purification - Rauch Tibor Attila
• 5. Biochemical analysis of purified protein - Rauch Tibor Attila
• 6. Physiological testing of purified protein - Rauch Tibor Attila
• 7. Oral presentation of a chosen topic - Rauch Tibor Attila
• 8. Oral presentation of a chosen topic - Rauch Tibor Attila

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Vom Institut veröffentlichter Lehrstoff

PowerPoint slides of the course

Skript

Empfohlene Literatur

Voraussetzung zum Absolvieren des Semesters

Test at the end of the semester

Semesteranforderungen

N/A

Möglichkeiten zur Nachholung der Fehlzeiten

N/A

Prüfungsfragen

1 The subject and history of genetic engineering

2 Ethical issues in genetic engineering

3 DNA, RNA and protein extraction from medical samples: past and present

4 Enzymes used in genetic engineering

5 Basics of molecular cloning

6 New genetic engineering technologies

7 Peptide- and protein-based therapies

8 Protein overproduction in prokaryotic and eukaryotic systems

9 Classical approach of antibody production

10 Production of antibodies with new technologies

11 Bispecific antibodies

12 CAR-T technology

13 Classical vaccine production

14 Recombinant viral vaccines

15 Recombinant RNA vaccines

16 Recombinant RNA- and DNA-based oncotherapy

Prüfer

Praktika, Seminarleiter/innen

• Rauch Tibor Attila