Medicine in the Future: Genetic Engineering and Biotechnology (Recombinant DNA/RNA/Protein Technologies)

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2024-2025

Tematika

Genetic engineering techniques offer unprecedented therapeutic possibilities that will certainly play a central role in future medicine. Today's oncotherapy and rheumatology toolkits already rely to a large extent on genetically engineered protein and peptide-based drugs. During the COVID-19 epidemic, recombinant viral and RNA vaccines proved their efficacy. In the light of these facts, future scientists and clinicians will benefit from this course, which will provide them a theoretical background of genetic engineering and practical insights into basic recombinant DNA, RNA and protein expression technologies. Current legal and medical positions on the subject will also be discussed.

Előadások

• 1. The subject and history of genetic engineering - Rauch Tibor Attila
• 2. Ethical issues in genetic engineering - Rauch Tibor Attila
• 3. DNA, RNA and protein extraction from medical samples: past and present - Rauch Tibor Attila
• 4. Enzymes used in genetic engineering - Rauch Tibor Attila
• 5. Basics of molecular cloning - Rauch Tibor Attila
• 6. New genetic engineering technologies - Rauch Tibor Attila
• 7. Protein overproduction in prokaryotic and eukaryotic systems - Rauch Tibor Attila
• 8. Peptide- and protein-based therapies - Rauch Tibor Attila
• 9. Classical approach of antibody production - Rauch Tibor Attila
• 10. Production of antibodies with new technologies - Rauch Tibor Attila
• 11. Bispecific antibodies - Rauch Tibor Attila
• 12. CAR-T technology - Rauch Tibor Attila
• 13. Classical vaccine production - Rauch Tibor Attila
• 14. Recombinant viral vaccines - Rauch Tibor Attila
• 15. Recombinant RNA vaccines - Rauch Tibor Attila
• 16. Recombinant RNA- and DNA-based oncotherapy - Rauch Tibor Attila

Gyakorlatok

• 1. RNA and DNA isolation from blood, PCR primer design - Rauch Tibor Attila
• 2. cDNA synthesis, PCR amplification - Rauch Tibor Attila
• 3. Cloning into protein expression vector - Rauch Tibor Attila
• 4. Recombinant protein overproduction and purification - Rauch Tibor Attila
• 5. Biochemical analysis of purified protein - Rauch Tibor Attila
• 6. Physiological testing of purified protein - Rauch Tibor Attila
• 7. Oral presentation of a chosen topic - Rauch Tibor Attila
• 8. Oral presentation of a chosen topic - Rauch Tibor Attila

Szemináriumok

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Saját oktatási anyag

PowerPoint slides of the course

Jegyzet

Ajánlott irodalom

A félév elfogadásának feltételei

Test at the end of the semester

Félévközi ellenőrzések

N/A

Távolmaradás pótlásának lehetőségei

N/A

Vizsgakérdések

1 The subject and history of genetic engineering

2 Ethical issues in genetic engineering

3 DNA, RNA and protein extraction from medical samples: past and present

4 Enzymes used in genetic engineering

5 Basics of molecular cloning

6 New genetic engineering technologies

7 Peptide- and protein-based therapies

8 Protein overproduction in prokaryotic and eukaryotic systems

9 Classical approach of antibody production

10 Production of antibodies with new technologies

11 Bispecific antibodies

12 CAR-T technology

13 Classical vaccine production

14 Recombinant viral vaccines

15 Recombinant RNA vaccines

16 Recombinant RNA- and DNA-based oncotherapy

Vizsgáztatók

Gyakorlatok, szemináriumok oktatói

• Rauch Tibor Attila