Molecular Toxicology - Practice

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

In the practices, the students will carry out laboratory experiments involving methods used for in vitro toxicity testing. On prokaryotic and eukaryotic cells, including cells of animal and human origin, they examine the cyto- and genotoxic effects of xenobiotics of toxicological relevance (e.g. mycotoxins and metal ions) and of UV radiation. Furthermore, protein-ligand interactions will be examined, including albumin binding and interactions with biotransformation enzymes. The students will be acquainted with the principles of the methods used, the mechanisms of action of the toxicants studied, the details of the experimental techniques, the statistical analysis as well as the presentation and interpretation of the experimental results. The laboratory work will be carried out in an interactive fashion. The students will perform the experiments jointly according to a detailed protocol previously provided for them.

Lectures

Practices

• 1.

  Investigation of flavonoid-albumin interaction I.

  - Dombi Ágnes
• 2.

  Investigation of flavonoid-albumin interaction II.

  - Dombi Ágnes
• 3.

  Investigation of flavonoid-albumin interaction III.

  - Dombi Ágnes
• 4.

  Investigation of flavonoid-albumin interaction IV.

  - Dombi Ágnes
• 5. Detection of UV-induced cyto- and genotoxicity in Serratia marcencens I - Hartnerné Pohóczky Krisztina
• 6. Detection of UV-induced cyto- and genotoxicity in Serratia marcencens II - Hartnerné Pohóczky Krisztina
• 7. Detection of UV-induced cyto- and genotoxicity in Serratia marcencens III - Hartnerné Pohóczky Krisztina
• 8. Detection of UV-induced cyto- and genotoxicity in Serratia marcencens IV - Hartnerné Pohóczky Krisztina
• 9. Detection of cytotoxicity by the Azotobacter agile assay I - Hartnerné Pohóczky Krisztina
• 10. Detection of cytotoxicity by the Azotobacter agile assay II - Hartnerné Pohóczky Krisztina
• 11. Detection of cytotoxicity by the Azotobacter agile assay III - Hartnerné Pohóczky Krisztina
• 12. Detection of cytotoxicity by the Azotobacter agile assay IV - Hartnerné Pohóczky Krisztina
• 13.

  Isolation of total RNA content of CHO cells using silica-membrane based spin column technology i

  - Hartnerné Pohóczky Krisztina
• 14.

  Isolation of total RNA content of CHO cells using silica-membrane based spin column technology II

  - Hartnerné Pohóczky Krisztina
• 15.

  Isolation of total RNA content of CHO cells using silica-membrane based spin column technology III

  - Hartnerné Pohóczky Krisztina
• 16.

  Isolation of total RNA content of CHO cells using silica-membrane based spin column technology IV

  - Hartnerné Pohóczky Krisztina
• 17.

  Changes in parameters of red and white wines after treatment with cyclodextrin polymer I.

  - Dombi Ágnes
• 18.

  Changes in parameters of red and white wines after treatment with cyclodextrin polymer II.

  - Dombi Ágnes
• 19.

  Changes in parameters of red and white wines after treatment with cyclodextrin polymer III.

  - Dombi Ágnes
• 20.

  Changes in parameters of red and white wines after treatment with cyclodextrin polymer IV.

  - Dombi Ágnes
• 21.

  Testing the kinetics of CYP2C9-catalyzed diclofenac hydroxylation I.

  - Dombi Ágnes
• 22.

  Testing the kinetics of CYP2C9-catalyzed diclofenac hydroxylation II.

  - Dombi Ágnes
• 23.

  Testing the kinetics of CYP2C9-catalyzed diclofenac hydroxylation III.

  - Dombi Ágnes
• 24.

  Testing the kinetics of CYP2C9-catalyzed diclofenac hydroxylation IV.

  - Dombi Ágnes
• 25.

  Testing the inhibitory effects of quercetin and allopurinol on xanthine oxidase-catalyzed uric acid formation I.

  - Dombi Ágnes
• 26.

  Testing the inhibitory effects of quercetin and allopurinol on xanthine oxidase-catalyzed uric acid formation II.

  - Dombi Ágnes
• 27.

  Testing the inhibitory effects of quercetin and allopurinol on xanthine oxidase-catalyzed uric acid formation III.

  - Dombi Ágnes
• 28.

  Testing the inhibitory effects of quercetin and allopurinol on xanthine oxidase-catalyzed uric acid formation IV.

  - Dombi Ágnes

Seminars

Reading material

Obligatory literature

Literature developed by the Department

A detailed description of each practice (handout) will be available for the students on Neptun in advance of each practice. The handout will explain the principle of the methods, characterize the toxicants to be tested, describe the necessary reagents and eqipment, the steps of the experiments (protocol), the presentation, statistical analysis and evaluation of the the results. The students are expected to review the handout thoroughly before taking part on the practice.

Notes

Recommended literature

C.D. Klassen (ed.): Casarett & Doull's Toxicology. The Basic Science of Poisons. 9th edition, McGraww-Hill, 2019.

Conditions for acceptance of the semester

-

Mid-term exams

The performance of students during laboratory practices will be evaluated and graded with a practical course mark, which will be based mainly on the quality of their written reports (laboratory notes) of each practice; however, their preparedness for the practices will also be considered.

Making up for missed classes

Missed classes cannot be made up.

Exam topics/questions

The performance of students during laboratory practices will be evaluated and graded with a practical course mark, which will be based mainly on the quality of their written reports (laboratory notes) of each practice; however, their preparedness for the practices will also be considered.

Examiners

Instructor / tutor of practices and seminars

• Dombi Ágnes
• Hartnerné Pohóczky Krisztina