Epifluorescence and Confocal Laser Scanning Microscopy in Biological Research

Data

Official data in SubjectManager for the following academic year: 2025-2026

Topic

The aim of this course is to familiarize students with the basic methods of biological research, including fluorescence microscopy techniques using epifluorescence and confocal laser scanning microscopy. The basic goal is to introduce the upper-year Bsc students of the University of Pécs to fluorescence microscopy techniques and their practical applications in different fields (e.g. medical research, medical diagnostics, food industry, environmental protection). Following the theoretical introductory lectures, the practical demonstration of confocal imaging microscopes in the centre of Nano-Bio-Imaging will be part of the course.

Lectures

• 1. Introduction to microscopy, history of microscopy - Szabó-Meleg Edina
• 2. Optical properties of light - Jánosi Tibor Zoltán
• 3. Resolution and magnification.Types and structures of fluorescent microscopes - Makkai Géza
• 4. Fluorescent molecules and proteins - Kovács Gergely
• 5. Epifluorescence and CLSM imaging - Kovács Gergely

Practices

• 1. Confocal laser scanning microscopy I. - Jánosi Tibor Zoltán
• 2. Confocal laser scanning microscopy II. - Jánosi Tibor Zoltán
• 3. Confocal laser scanning microscopy III. - Jánosi Tibor Zoltán
• 4. Confocal laser scanning microscopy IV. - Jánosi Tibor Zoltán
• 5. STED microscopy I. - Makkai Géza
• 6. STED microscopy II. - Makkai Géza
• 7. STED microscopy III. - Makkai Géza

Seminars

Reading material

Obligatory literature

Jerome WG and Price RL: Basic Confocal Microscopy, Spinger, 2018

Literature developed by the Department

Notes

Lecture notes

Recommended literature

Conditions for acceptance of the semester

Written exam

Mid-term exams

Written exam

Making up for missed classes

Upon individual aggrement

Exam topics/questions

-

Examiners

Instructor / tutor of practices and seminars

• Jánosi Tibor Zoltán
• Makkai Géza