Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
-
Kovács Gergely
senior research fellow,
Department of Dermatology, Venereology and Oncodermatology -
Number of hours/semester
lectures: 12 hours
practices: 0 hours
seminars: 0 hours
total of: 12 hours
Subject data
- Code of subject: OTF-EFL-T
- 1 kredit
- Biotechnology BSc
- Optional modul
- spring
OTN-BFB2-T finished
Course headcount limitations
min. 5 – max. 6
Topic
The aim of this course is to familiarize students with the basic methods of biological research, including fluorescence microscopy techniques using epifluorescence and confocal laser scanning microscopy. The basic goal is to introduce the upper-year Bsc students of the University of Pécs to fluorescence microscopy techniques and their practical applications in different fields (e.g. medical research, medical diagnostics, food industry, environmental protection). Following the theoretical introductory lectures, the practical demonstration of confocal imaging microscopes in the centre of Nano-Bio-Imaging will be part of the course.
Lectures
- 1. Introduction to microscopy, history of microscopy - Kovács Gergely
- 2. Optical properties of light - Kovács Gergely
- 3. Resolution and magnification - Kovács Gergely
- 4. Fluorescent molecules and proteins - Kovács Gergely
- 5. Types and structure of fluorescence microscopes - Kovács Gergely
- 6. Epifluorescence imaging - Kovács Gergely
- 7. Confocal laser scanning microscopy I. - Kovács Gergely
- 8. Confocal laser scanning microscopy II. - Kovács Gergely
- 9. Confocal laser scanning microscopy III. - Kovács Gergely
- 10. Super-resolution microscopy techniques I. - Kovács Gergely
- 11. Super-resolution microscopy techniques II. - Kovács Gergely
- 12. Super-resolution microscopy techniques III. - Kovács Gergely
Practices
Seminars
Reading material
Obligatory literature
Jerome WG and Price RL: Basic Confocal Microscopy, Spinger, 2018
Literature developed by the Department
Notes
Lecture notes
Recommended literature
Conditions for acceptance of the semester
Written exam
Mid-term exams
Written exam
Making up for missed classes
Upon individual aggrement
Exam topics/questions
-