Epifluorescence and Confocal Laser Scanning Microscopy in Biological Research

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The aim of this course is to familiarize students with the basic methods of biological research, including fluorescence microscopy techniques using epifluorescence and confocal laser scanning microscopy. The basic goal is to introduce the upper-year Bsc students of the University of Pécs to fluorescence microscopy techniques and their practical applications in different fields (e.g. medical research, medical diagnostics, food industry, environmental protection). Following the theoretical introductory lectures, the practical demonstration of confocal imaging microscopes in the centre of Nano-Bio-Imaging will be part of the course.

Lectures

• 1. Introduction to microscopy, history of microscopy - Kovács Gergely
• 2. Optical properties of light - Kovács Gergely
• 3. Resolution and magnification - Kovács Gergely
• 4. Fluorescent molecules and proteins - Kovács Gergely
• 5. Types and structure of fluorescence microscopes - Kovács Gergely
• 6. Epifluorescence imaging - Kovács Gergely
• 7. Confocal laser scanning microscopy I. - Kovács Gergely
• 8. Confocal laser scanning microscopy II. - Kovács Gergely
• 9. Confocal laser scanning microscopy III. - Kovács Gergely
• 10. Super-resolution microscopy techniques I. - Kovács Gergely
• 11. Super-resolution microscopy techniques II. - Kovács Gergely
• 12. Super-resolution microscopy techniques III. - Kovács Gergely

Practices

Seminars

Reading material

Obligatory literature

Jerome WG and Price RL: Basic Confocal Microscopy, Spinger, 2018

Literature developed by the Department

Notes

Lecture notes

Recommended literature

Conditions for acceptance of the semester

Written exam

Mid-term exams

Written exam

Making up for missed classes

Upon individual aggrement

Exam topics/questions

-

Examiners

Instructor / tutor of practices and seminars