Epifluorescence and Confocal Laser Scanning Microscopy in Biological Research

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2024-2025

Thematik

The aim of this course is to familiarize students with the basic methods of biological research, including fluorescence microscopy techniques using epifluorescence and confocal laser scanning microscopy. The basic goal is to introduce the upper-year Bsc students of the University of Pécs to fluorescence microscopy techniques and their practical applications in different fields (e.g. medical research, medical diagnostics, food industry, environmental protection). Following the theoretical introductory lectures, the practical demonstration of confocal imaging microscopes in the centre of Nano-Bio-Imaging will be part of the course.

Vorlesungen

• 1. Introduction to microscopy, history of microscopy - Kovács Gergely
• 2. Optical properties of light - Kovács Gergely
• 3. Resolution and magnification - Kovács Gergely
• 4. Fluorescent molecules and proteins - Kovács Gergely
• 5. Types and structure of fluorescence microscopes - Kovács Gergely
• 6. Epifluorescence imaging - Kovács Gergely
• 7. Confocal laser scanning microscopy I. - Kovács Gergely
• 8. Confocal laser scanning microscopy II. - Kovács Gergely
• 9. Confocal laser scanning microscopy III. - Kovács Gergely
• 10. Super-resolution microscopy techniques I. - Kovács Gergely
• 11. Super-resolution microscopy techniques II. - Kovács Gergely
• 12. Super-resolution microscopy techniques III. - Kovács Gergely

Praktika

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Jerome WG and Price RL: Basic Confocal Microscopy, Spinger, 2018

Vom Institut veröffentlichter Lehrstoff

Skript

Lecture notes

Empfohlene Literatur

Voraussetzung zum Absolvieren des Semesters

Written exam

Semesteranforderungen

Written exam

Möglichkeiten zur Nachholung der Fehlzeiten

Upon individual aggrement

Prüfungsfragen

-

Prüfer

Praktika, Seminarleiter/innen