Biophysics - Lecture

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2022-2023

Tematika

The Biophysics curse aims to introduce the students to the methods and applications, which are routinely used in both medical and pharmaceutical biotechnology. The principles of the state-of-the-art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR) imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI), radioactive applications and calorimetric and fast kinetics techniques. The lectures discuss in detail the physical basis and principles of each approach and the field of applications. The practicals are dedicated to extending the students' knowledge and routine in the use of the different techniques. The practicals lay special emphasis on presenting not only the routine applications but advanced uses of each technique. The limitations, as well as the artifacts that can be caused by improper experimental planning, are highlighted.

Előadások

• 1. Introduction: biophysics in biotechnology. - Dr. Bugyi Beáta
• 2. Databases, resources. - Dr. Bugyi Beáta
• 3. Electromagnetic waves, the electromagnetic spectrum. - Dr. Grama László
• 4. The wave and particle nature of light. - Dr. Grama László
• 5. Energy levels of atoms and molecules. - Dr. Lukács András Szilárd
• 6. Laser. - Dr. Lukács András Szilárd
• 7. UV-VIS spectroscopy. Circular dichroism (CD) spectroscopy. - Dr. Huber Tamás
• 8. Infrared and Raman spectroscopy. - Dr. Bugyi Beáta
• 9. Fluorescence spectroscopy, fluorescence parameters. Förster resonance energy transfer (FRET). - Dr. Huberné Dr. Barkó Szilvia
• 10. Flow cytometry. Fluorescence activated cell sorting. - Dr. Huberné Dr. Barkó Szilvia
• 11. The spin, nuclear magnetic resonance (NMR) spectroscopy. - Dr. Grama László
• 12. Magnetic resonance imaging (MRI). - Dr. Grama László
• 13. Basics of light microscopy and fluorescence microscopy. - Dr. Bugyi Beáta
• 14. Advanced fluorescence microscopy 1. - Dr. Bugyi Beáta
• 15. Advanced fluorescence microscopy 2. - Dr. Bugyi Beáta
• 16. Image analysis. - Dr. Bugyi Beáta
• 17. Rapid kinetic methods: stopped-flow, surface plasmon resonance (SPR). - Dr. Kengyel András Miklós
• 18. Sound. Ultrasound. - Dr. Kengyel András Miklós
• 19. Radioactivity, the interaction of radioactive radiations with the matter. - Dr. Szabó-Meleg Edina
• 20. Dosimetry, detection of radioactive radiations. - Dr. Szabó-Meleg Edina
• 21. Biological effects of radioactive radiations. - Dr. Kengyel András Miklós
• 22. Gamma-camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET). - Dr. Kengyel András Miklós
• 23. Thermodynamics: laws, thermodynamic potentials. - Dr. Lukács András Szilárd
• 24. Calorimetric methods: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC). - Dr. Lukács András Szilárd
• 25. Analytical separation techniques 1: sedimentation, electrophoresis. - Dr. Bukovics Péter
• 26. Analytical separation techniques 2: chromatography. - Dr. Bukovics Péter
• 27. Mass spectrometry. - Dr. Talián Csaba Gábor
• 28. X-ray diffraction. - Dr. Talián Csaba Gábor

Gyakorlatok

Szemináriumok

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Saját oktatási anyag

Homepage of the Department of Biophysics:
https://aok.pte.hu/en/egyseg/10/oktatasi-anyagok

Jegyzet

Ajánlott irodalom

A félév elfogadásának feltételei

Maximum of 15 % absence allowed

Félévközi ellenőrzések

Theory exams are scheduled in the exam period according to the rules of studies and examinations.

Távolmaradás pótlásának lehetőségei

The opportunity to make up for absence is not provided.

Vizsgakérdések

Topics of the exam questions:
Electromagnetic waves
Quantum numbers, NMR, MRI
Energy levels of atoms and molecules
Laser
UV-VIS absorption spectroscopy
Fluorescence spectroscopy
Infrared and Raman spectroscopy
Rapid kinetic methods
Flow cytometry
Light and fluorescence microscopy
Advanced fluorescence microscopy
Image analysis
Sound, ultrasound
Gamma camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET)
Radioactivity, the interaction of radioactive radiations with matter
Biological effects of radioactive radiations, dosimetry
X-ray diffraction
Thermodynamics: laws and thermodynamic potentials
Calorimetry: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC)
Analytical separation techniques: sedimentation, electrophoresis
Analytical separation techniques: chromatographic techniques
Mass spectrometry

Vizsgáztatók

• Dr. Bugyi Beáta
• Dr. Bukovics Péter
• Dr. Grama László
• Dr. Kengyel András Miklós
• Dr. Lukács András Szilárd
• Dr. Szabó-Meleg Edina
• Dr. Talián Csaba Gábor

Gyakorlatok, szemináriumok oktatói