Adatok
A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2020-2021
Tantárgyfelelős
-
Dr. Tímea BENCSIK
assistant professor,
Department of Pharmacognosy
Óraszámok/félév
előadás: 28 óra
gyakorlat: 0 óra
szeminárium: 0 óra
összesen: 28 óra
Tárgyadatok
- Kód: OPF-EXT-T
- 2 kredit
- Pharmacy
- Optional modul
- spring
OPA-M1E-T completed
Kurzus létszámkorlát
min. 5 fő – max. 20 fő
Tematika
With the increasing demand for herbal medicinal products, nutraceuticals, and natural products for health care all over the world, medicinal plant extract manufacturers and essential oil producers have started using the most appropriate extraction and chromatographic technologies in order to produce and standardize extracts and essential oils of defined quality. The aim of this course is to introduce the most important extraction methods and chromatographic techniques used in the research of medicinal plants. Students should be able to apply the acquired knowledge in their medical or pharmaceutical practice.
Task: To introduce state-of-the-art extraction techniques (maceration, infusion, digestion, decoction, percolation, Soxhlet, ultrasound extraction, supercritical fluid extraction, water and steam distillation, enfleurage, expression) and chromatographic analyses (column chromatography, thin layer chromatography, high pressure liquid chromatography) of medicinal plants and their role in the production of herbal medicines. Some applications, relating to different classes of substances, are presented to demonstrate the versatility of various chromatographic techniques.
Requirements: students have to know the most frequent extraction and analytical techniques used in medicinal and aromatic plant research.
Előadások
- 1. Introduction of the course requirements. An Overview of Extraction Techniques for Medicinal and Aromatic Plants. - Dr. Bencsik Tímea
- 1. Chromatographic seminar: TLC. - Dr. Benkő András Antal
- 2. Introduction of state-of-the-art extraction techniques: maceration, infusion, digestion, decoction, percolation, Soxhlet, ultrasound extraction, supercritical fluid extraction, water and steam distillation, enfleurage, expression. - Dr. Bencsik Tímea
- 2. Chromatographic seminar: HPLC. - Dr. Bencsik Tímea
- 3. Introduction to Analytical Separations. - Dr. Benkő András Antal
- 4. Techniques for the Purposes of Botanical Research. - Dr. Benkő András Antal
- 5. Planar Chromatography: TLC. - Dr. Benkő András Antal
- 6. Role of Different Procedures and Test Systems in the Knowledge of Herbal Substances. - Dr. Benkő András Antal
- 7. Column Chromatography Systems: GC. - Dr. Benkő András Antal
- 8. Column Chromatography Systems: HPLC. - Dr. Benkő András Antal
- 9. Detectors Used in Different Separation Techniques and their Role in Qualitative and Quantitative Analysis. - Dr. Benkő András Antal
- 10. Classification of Detectors Based on their Sensitivity and Selectivity. - Dr. Benkő András Antal
- 11. Role of thin layer chromatography (TLC) in detecting microbiological processes: TLC-Bioautography. - Dr. Horváth Györgyi
- 12. Antimicrobial activity of essential oils using TLC-Bioautography. - Dr. Horváth Györgyi
- 13. Isolation and identification of carotenoids from medicinal plants. - Dr. Molnár Péter
- 14. Role of column liquid chromatography (CLC) in carotenoid research. - Dr. Molnár Péter
- 15. Examination of honeys, sugars, polyphenols. - Dr. Farkas Ágnes
- 16. Examination of honeys, sugars, polyphenols. - Dr. Farkas Ágnes
- 17. Identification of specific metabolites in herbal products. Adulterated dietary supplements. - Dr. Bencsik Tímea
- 18. Identification of specific metabolites in herbal products with TLC. - Dr. Bencsik Tímea
- 19. Isolation and Identification of flavonoids in medicinal plants. - Dr. Papp Nóra
- 20. Isolation and Identification of flavonoids in medicinal plants. - Dr. Papp Nóra
- 21. Quality of herbal products; our experiences in the Department of Pharmacognosy. - Dr. Bencsik Tímea
- 22. Quality of herbal products; our experiences in the Department of Pharmacognosy. - Dr. Bencsik Tímea
- 23. Student's lecture. - Dr. Bencsik Tímea
- 24. Student's lecture. - Dr. Bencsik Tímea
- 25. Written test. - Dr. Bencsik Tímea
- 26. Written test. - Dr. Bencsik Tímea
Gyakorlatok
Szemináriumok
A tananyag elsajátításához szükséges segédanyagok
Kötelező irodalom
Saját oktatási anyag
PowerPoint presentations prepared by lecturers.
Jegyzet
Ajánlott irodalom
1. Handa S.S., Khanuja S.P.S., Longo G., Rakesh D.D.: Extraction Technologies for Medicinal and Aromatic Plants, International Centre for Science and High Technology, Trieste, 2008
2. Reich E., Schibli A.: High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants, Thieme, UK, 2007
3. Colegate S.M.: Bioactive Natural Products Detection Isolation and Structural Determination, CRC Press, 2008
4. Niessen W.M.A.: Liquid Chromatography - Mass Spectrometry, CRC Press, 2006
5. Nyiredy, Sz.: Planar Chromatography, Springer, Budapest, 2001.
6. Harris, DC.: Quantitative Chemical Analysis, WH. Freeman and Co., 7th edition, New York, 2007,
7. Schiedt K., Liaaen-Jensen S.: Isolation and Analysis, in Carotenoids Vol. 1A: Isolation and Analysis, Eds.: Britton G., Liaaen-Jensen S., Pfander H., Birkhäuser Verlag, Basel, 1995.
A félév elfogadásának feltételei
Maximum of 25 % absence allowed
Félévközi ellenőrzések
One written exam during the semester, the acceptance level is 60%. One oral presentation by students.
Távolmaradás pótlásának lehetőségei
There is no possibility to make up for a missed lecture and practice.
Vizsgakérdések
The topics of the 1 written test are similar to the topics of the lectures.
Vizsgáztatók
Gyakorlatok, szemináriumok oktatói
- Dr. Bencsik Tímea
- Dr. Benkő András Antal