Advanced Tissue Culture Technologies - Lecture

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2026-2027

Tematika

The course material is designed to provide theoretical background to currently employed three dimensional tissue culture techniques including scaffold-based (biodegradable) and scaffold-free (tissue printing, pelleting and hanging-drop) systems. The course will detail the importance of the tissue microenvironment in normal and artificial tissue development. Directed cellular growth, organ formation for surgical implantation and drug target identification in human tissue systems and in vitro testing of pharmaceutical compounds in complex tissue systems are also part of the curriculum. The course will discuss the latest advances in technology and medicine for replacing tissues and organs damaged by disease and of developing therapies for previously untreatable conditions.

Előadások

• 1. Introduction, basic principles of tissue engineering (TE) - Pongrácz Judit Erzsébet
• 2. 2D and 3D cell and tissue culture - Pongrácz Judit Erzsébet
• 3. Cells and tissue types in tissue engineering I: stem cell types, their characteristics and potential applications (hESCs, iPSCs, fetal stem cells) - Pongrácz Judit Erzsébet
• 4. Cells and tissue types in tissue engineering II: stem cell types, their characteristics and potential applications (adult somatic stem cells, eg. ADSCs, dental stem cells). Differentiated, organ-specific primary cells. - Pongrácz Judit Erzsébet
• 5. Bioreactors, requirements of bioreactor design. Characteristics and use of the main bioreactor types I. - Pongrácz Judit Erzsébet
• 6. Characteristics and use of the main bioreactor types II. - Pongrácz Judit Erzsébet
• 7. Scaffolds in tissue engineering. Types and characteristics of frequently used biomaterials I. - Pongrácz Judit Erzsébet
• 8. Types and characteristics of frequently used biomaterials II. - Pongrácz Judit Erzsébet
• 9. Degradation of polymer scaffolds. Technologies of scaffold fabrication I. - Pongrácz Judit Erzsébet
• 10. Technologies of scaffold fabrication II. - Pongrácz Judit Erzsébet
• 11. Cell-scaffold interactions. Scaffold parameters affecting cell behavior. - Pongrácz Judit Erzsébet
• 12. Biofactors in TE. Controlled release of bioactive factors in engineered tissues. Drug delivery systems in TE I. - Pongrácz Judit Erzsébet
• 13. Drug delivery systems in TE II. Biosensors in TE. - Pongrácz Judit Erzsébet
• 14. Types and use of aggregate cultures. Organoids and spheroid cultures. - Pongrácz Judit Erzsébet
• 15. Technologies used for the generation of aggregate cultures I. - Pongrácz Judit Erzsébet
• 16. Technologies used for the generation of aggregate cultures II. Microfluidic systems. - Pongrácz Judit Erzsébet
• 17. Advanced tissue models using transwell inserts: Air-Liquid Interface (ALI) technology and Blood-Brain-Barrier (BBB) models. - Pongrácz Judit Erzsébet
• 18. Test - Pongrácz Judit Erzsébet
• 19. Tumor models I. - Pongrácz Judit Erzsébet
• 20. Tumor models II. - Pongrácz Judit Erzsébet
• 21. Tissue printing technologies I. - Pongrácz Judit Erzsébet
• 22. Tissue printing technologies II. - Pongrácz Judit Erzsébet
• 23. Tissue engineering for transplantation I. Timeline and milestones of organ and tissue transplantation - Pongrácz Judit Erzsébet
• 24. Hematopoietic stem cell transplantation. - Pongrácz Judit Erzsébet
• 25. Tissue engineering for transplantation I. TE of pancreatic islets - Pongrácz Judit Erzsébet
• 26. Tissue engineering for transplantation II. Cartilage TE. - Pongrácz Judit Erzsébet
• 27. Tissue engineering for transplantation II. Skin substitutes. - Pongrácz Judit Erzsébet
• 28. Types and regulation of advanced therapy medicinal products (ATMPs). - Pongrácz Judit Erzsébet

Gyakorlatok

Szemináriumok

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Saját oktatási anyag

Lecture ppt

Jegyzet

Three dimensional tissue cultures and tissue engineering -access via www.gytk.hu

Three dimensional tissue cultures and tissue engineering - access via www.tankonyvtar.hu

Ajánlott irodalom

Pongracz & Keen: Medical Biotechnology, Elsevier, 2009

Joseph D. Bronzino: Tissue Engineering and Artificial Organs

Ali Khademhosseini (ed.): Micro- and Nanoengineering of the Cell Microenvironment, Technologies and Applications (Engineering in Medicine & Biology)

Challa S. S. R. Kumar (ed.): Tissue, Cell and Organ Engineering (Nanotechnologies for the Life Sciences)

W. Mark Saltzman: Tissue Engineering: Engineering Principles for the Design of Replacement Organs and Tissues

Robert Lanza, Robert Langer, Joseph Vacanti: Principles of Tissue Engineering, 3rd edition

Will W. Minuth, Raimund Strehl, Karl Schumacher: Tissue Engineering: From Cell Biology to Artificial Organs

Fen Ontaigne: Medicine by Design: The Practice and Promise of Biomedical Engineering, Forgacs & Newman, 2005.

A félév elfogadásának feltételei

75% attendance to lectures and completion of the midterm test, then final exam

Félévközi ellenőrzések

Midterm test

Távolmaradás pótlásának lehetőségei

None

Vizsgakérdések

Written test. Online Unipol system, Teams, Moodle

Vizsgáztatók

• Bóvári-Biri Judit
• Pongrácz Judit Erzsébet

Gyakorlatok, szemináriumok oktatói

• Bóvári-Biri Judit
• Papp Alexandra