Possible Applications of Spectroscopic Methods Regarding the Investigation of Reversible Interactions

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2022-2023

Tematika

Optical spectroscopic methods are well suited for the study of reversible interactions (e.g., protein-ligand) of drugs, nutrients, toxins, and other xenobiotics. During the course, students will get acquainted with the theoretical and practical applications of widely used UV-visible absorption, fluorescence emission, and fluorescence anisotropy measurements, and will then learn the use of these methods in the study of various reversible interactions through concrete examples. The knowledge gained in this way promotes the understanding of measurement techniques and evaluation options and develops students' problem-solving skills and creativity in connection with spectroscopic examinations.

Előadások

• 1. Description of the course requirements. Introduction: Optical spectroscopic methods. - Dr. Lemli Beáta
• 2. Theory of UV-visible spectrophotometry - Dr. Lemli Beáta
• 3. UV-visible spectrophotometry: examples of application - Dr. Lemli Beáta
• 4. Theory of fluorescence spectroscopy I. - Dr. Lemli Beáta
• 5. Theory of fluorescence spectroscopy II. - Dr. Lemli Beáta
• 6. Theory of fluorescence spectroscopy III. - Dr. Lemli Beáta
• 7. Theory of fluorescence spectroscopy IV. - Dr. Lemli Beáta
• 8. Characteristics of fluorescence emission: examples of application I. - Dr. Lemli Beáta
• 9. Characteristics of fluorescence emission: examples of application II. - Dr. Lemli Beáta
• 10. Fluorescence anisotropy: examples of application I. - Dr. Lemli Beáta
• 11. Fluorescence anisotropy: examples of application II. - Dr. Lemli Beáta
• 12. Summary and conclusions - Dr. Lemli Beáta
• 13. Written test - Dr. Lemli Beáta

Gyakorlatok

• 1. General instructions for work in the laboratory, safety precautions.
• 2. UV-visible spectrophotometric measurement
• 3. UV-visible spectrophotometric measurement
• 4. UV-visible spectrophotometric measurement
• 5. UV-visible spectrophotometric measurement
• 6. Fluorescence spectroscopic measurement - fluorescence emission
• 7. Fluorescence spectroscopic measurement - fluorescence emission
• 8. Fluorescence spectroscopic measurement - fluorescence emission
• 9. Fluorescence spectroscopic measurement - fluorescence emission
• 10. Fluorescence spectroscopic measurement - anisotropy
• 11. Fluorescence spectroscopic measurement - anisotropy
• 12. Fluorescence spectroscopic measurement - anisotropy
• 13. Fluorescence spectroscopic measurement - anisotropy

Szemináriumok

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Joseph R. Lakowicz: Principles of Fluorescence Spectroscopy, e.g. 3rd edition eBook ISBN 978-0-387-46312-4

Saját oktatási anyag

The slides of the lectures are electronically available.

Jegyzet

Ajánlott irodalom

Joseph R. Lakowicz: Principles of Fluorescence Spectroscopy, e.g. 3rd edition eBook ISBN 978-0-387-46312-4

A félév elfogadásának feltételei

Maximum of 15 % absence allowed

Félévközi ellenőrzések

One written exam on the 13th lecture, the acceptance level is 60%. The topics of the written test are similar to the topics of the lectures. An accepted report about the practacies (lab book).

Távolmaradás pótlásának lehetőségei

There is no possibility to make up for a missed lecture and/or practice.

Vizsgakérdések

The topics of the written test are similar to the topics of the lectures.

Vizsgáztatók

Gyakorlatok, szemináriumok oktatói

• Dr. Lemli Beáta