Molecular Diagnostics

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The modern laboratory diagnostic methods, including molecular and immunological techniques will be discussed.

Lectures

• 1. Basic terms of immunologic detection methods. Antigen-antibody reactions. Sensitivity, specificity of antigen-antibody reactions. Immunoassay types. - Berki Timea
• 2. Multiplex detection methods. Cytokine, chemokine detection methods. Biomarkers. - Berki Timea
• 3. Immunserological methods, autoantibody testing, detection of allergen specific IgE. - Simon Diána
• 4. The most frequently used analytical methods. Preanalytics, postanalytics. Laboratory automation. - Nagy Tamás
• 5. Plasma protein testing methods and evaluation of results in laboratory medicine. - Nagy Tamás
• 6. HLA and disease assotiation, methods for HLA-typing, HLA-B27 typing. Phage display. - Simon Diána
• 7. Molecular genetics in laboratory medicine - Gombos Katalin
• 8. Methods for locus-specific and genome-wide epigenetic studies - Rauch Tibor Attila
• 9. Genetic diagnostics of Mendelian disorders - Berenténé Bene Judit Ágnes
• 10. DNA and RNA sequencing technologies and applications - Urbán Péter
• 11. Molecular diagnostics of microbes. Nucleic Acid-based Techniques (NATs) in pathogen detection. - Pankovics Péter
• 12. Molecular diagnostics of microbes. Molecular typing methods in multi-drug resistant bacterial outbreak investigations. - Pál Ágnes Mária (Sonnevend)
• 13. Cytogenetics in oncological diagnostics - Kajtár Béla
• 14. Molecular methods in precision oncology - Kajtár Béla

Practices

Seminars

• 1. Antigen-antibody reactions. Immunoassay types.
• 2. Multiplex detection methods
• 3. Autoantibody detection methods. Diagnostics of systemic autoimmune diseases. Laboratory diagnostics of allergy.
• 4. Immunchemistry laboratory equipments, detection methods.
• 5. Protein chemistry
• 6. HLA and diseases. HlA typing. Phage display libraries.
• 7. Genetics and molecular biology in laboratory medicine
• 8. Methods for epigenetic studies
• 9. Basic terms of genetic diagnostics. Genetic diagnostics of Mendelian disorders.
• 10. DNA and RNA sequencing technologies
• 11. Molecular diagnostics of microbes.
• 12. Molecular diagnostics of microbes
• 13. Cytogenetics in oncological diagnostics
• 14. Molecular testing in oncology

Reading material

Obligatory literature

Literature developed by the Department

www.medbiotech.com

Notes

Recommended literature

Conditions for acceptance of the semester

Attendance at lectures and active participation in seminars.

Mid-term exams

-

Making up for missed classes

None

Exam topics/questions

Written test exam with questions provided by the lecturers, if necessary in online format (moodle).

Examiners

• Simon Diána

Instructor / tutor of practices and seminars

• Berenténé Bene Judit Ágnes
• Berki Timea
• Gombos Katalin
• Kajtár Béla
• Nagy Tamás
• Pál Ágnes Mária (Sonnevend)
• Pankovics Péter
• Rauch Tibor Attila
• Simon Diána
• Urbán Péter