Molecular Diagnostics

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2024-2025

Tematika

The modern laboratory diagnostic methods, including molecular and immunological techniques will be discussed.

Előadások

• 1. Basic terms of immunologic detection methods. Antigen-antibody reactions. Sensitivity, specificity of antigen-antibody reactions. Immunoassay types. - Berki Timea
• 2. Multiplex detection methods. Cytokine, chemokine detection methods. Biomarkers. - Berki Timea
• 3. Immunserological methods, autoantibody testing, detection of allergen specific IgE. - Simon Diána
• 4. The most frequently used analytical methods. Preanalytics, postanalytics. Laboratory automation. - Nagy Tamás
• 5. Plasma protein testing methods and evaluation of results in laboratory medicine. - Nagy Tamás
• 6. HLA and disease assotiation, methods for HLA-typing, HLA-B27 typing. Phage display. - Simon Diána
• 7. Molecular genetics in laboratory medicine - Gombos Katalin
• 8. Methods for locus-specific and genome-wide epigenetic studies - Rauch Tibor Attila
• 9. Genetic diagnostics of Mendelian disorders - Berenténé Bene Judit Ágnes
• 10. DNA and RNA sequencing technologies and applications - Urbán Péter
• 11. Molecular diagnostics of microbes. Nucleic Acid-based Techniques (NATs) in pathogen detection. - Pankovics Péter
• 12. Molecular diagnostics of microbes. Molecular typing methods in multi-drug resistant bacterial outbreak investigations. - Pál Ágnes Mária (Sonnevend)
• 13. Cytogenetics in oncological diagnostics - Kajtár Béla
• 14. Molecular methods in precision oncology - Kajtár Béla

Gyakorlatok

Szemináriumok

• 1. Antigen-antibody reactions. Immunoassay types.
• 2. Multiplex detection methods
• 3. Autoantibody detection methods. Diagnostics of systemic autoimmune diseases. Laboratory diagnostics of allergy.
• 4. Immunchemistry laboratory equipments, detection methods.
• 5. Protein chemistry
• 6. HLA and diseases. HlA typing. Phage display libraries.
• 7. Genetics and molecular biology in laboratory medicine
• 8. Methods for epigenetic studies
• 9. Basic terms of genetic diagnostics. Genetic diagnostics of Mendelian disorders.
• 10. DNA and RNA sequencing technologies
• 11. Molecular diagnostics of microbes.
• 12. Molecular diagnostics of microbes
• 13. Cytogenetics in oncological diagnostics
• 14. Molecular testing in oncology

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Saját oktatási anyag

www.medbiotech.com

Jegyzet

Ajánlott irodalom

A félév elfogadásának feltételei

Attendance at lectures and active participation in seminars.

Félévközi ellenőrzések

-

Távolmaradás pótlásának lehetőségei

None

Vizsgakérdések

Written test exam with questions provided by the lecturers, if necessary in online format (moodle).

Vizsgáztatók

• Simon Diána

Gyakorlatok, szemináriumok oktatói

• Berenténé Bene Judit Ágnes
• Berki Timea
• Gombos Katalin
• Kajtár Béla
• Nagy Tamás
• Pál Ágnes Mária (Sonnevend)
• Pankovics Péter
• Rauch Tibor Attila
• Simon Diána
• Urbán Péter