Urinary Steroid Profiles

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2024-2025

Tematika

The participants are going to be familiarised theoretically and practically with:

- pre-treatment of urine sample

- qualitative and quantitative analysis of 24 androgen, progesterone and corticoid metabolites by gas chromatography using mass selective detector

- evaluation of urinary steroid profiles

Előadások

• 1. Urinary steroid metabolites - Bufa Anita
• 2. Pre-treatment of urine sample - Bufa Anita
• 3. Gas chromatography-Mass spectrometry - Bufa Anita
• 4. Qualitative analysis of urinary steroid metabolites - Bufa Anita
• 5. Quantitative analysis of urinary steroid metabolites - Bufa Anita
• 6. Evaluation of urinary steroid profile - Bufa Anita

Gyakorlatok

• 1. Solid-phase extraction of urinary steroid metabolites - Bufa Anita
• 2. Enzymatic hydrolysis of urinary steroid metabolites - Bufa Anita
• 3. Derivatization of urinary steroid metabolites - Bufa Anita
• 4. Chromatographic cleaning - Bufa Anita
• 5. Determination of urinary steroid profiles by GC-MS technic - Bufa Anita
• 6. Qualitative and quantitative analysis of urinary steroid metabolites - Bufa Anita

Szemináriumok

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Saját oktatási anyag

It is going to be available on the website of Teams group and PotePedia.

Jegyzet

Ajánlott irodalom

A félév elfogadásának feltételei

After the course a written essay.

Félévközi ellenőrzések

After the course a written essay.

Távolmaradás pótlásának lehetőségei

Consultation with course director is required. The necessary information is going to be presented on the course.

Vizsgakérdések

The necessary information is going to be presented on the course.

Vizsgáztatók

Gyakorlatok, szemináriumok oktatói

• Bufa Anita