Adatok
A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2024-2025
Tantárgyfelelős
-
Makszin Lilla
assistant professor,
Institute of Bioanalysis
Óraszámok/félév
előadás: 10 óra
gyakorlat: 12 óra
szeminárium: 0 óra
összesen: 22 óra
Tárgyadatok
- Kód: OPF-CTD-T
- 2 kredit
- Pharmacy
- Optional modul
- autumn
Nincs
Vizsgakurzus:Kurzus létszámkorlát
min. 5 fő – max. 15 fő
Campus kurzusként elérhető . Campus-karok: ETK TTK
Tematika
Microchip capillary electrophoresis is an interesting approach for an increasing number of analytical problems in environmental applications and health science. The determination of size, quality and concentration of biomolecules such as DNA, RNA and proteins is one of the fundamental steps in life science research. Traditional methods for this type of analysis, such as gel electrophoresis or capillary electrophoresis, can now be complemented by an analytical technique, Lab-on-a-Chip technology. This technology enables downscaling and integration of several experimental steps (injection, labeling, dilution, separation, detection) into one process, in combination with automated data analysis. Lab-on-a-Chip technology has several advantages compared with conventional techniques, such as minimal sample requirement, rapid analysis times, ease-of-use, minimized exposure to hazardous materials and reduced waste generation.
The course deals with the theoretical background and application of the microfluidic methods, such as electrophoresis for detection of proteins, endotoxins, DNA, RNA and flow cytometry for detection of cells (bacteria, fungi, cancer cells, etc.).
Előadások
- 1. The principle and practice of capillary zone electrophoresis, theory of electrophoretic migration - Makszin Lilla
- 2. The principle and practice of gel electrophoresis, polyacrylamide gel electrophoresis (2-D PAGE, SDS-PAGE) methods - Makszin Lilla
- 3. The principle and practice of microchip electrophoresis - Makszin Lilla
- 4. The principle and practice of flow cytometry on microchip - Makszin Lilla
- 5. The structure and function of microchip electrophoresis device (Agilent Bioanalyzer 2100) (different types of detectors, injection methods) - Makszin Lilla
- 6. Preparation of microfluidic chips, properties and advantages - Makszin Lilla
- 7. Laboratory diagnostic applications of microfluidic chips 1. (electrophoretic separation of proteins, DNA, RNA) - Makszin Lilla
- 8. Laboratory diagnostic applications of microfluidic chips 2: (flow cytometry analysis of bacteria, fungi, cancer cells) - Makszin Lilla
- 9. Qualitative evaluation of „lab-on-a-chip” methods by help of Agilent 2100 Expert software - Makszin Lilla
- 10. Quantitative evaluation of „lab-on-a-chip” methods by help of Agilent 2100 Expert software - Makszin Lilla
Gyakorlatok
- 1. Preparation of samples (serum proteins, bacterial proteins, endotoxins, etc.) - Makszin Lilla
- 2. Preparation of samples; covalent labeling with fluorescent dye - Makszin Lilla
- 3. Preparation of samples; denaturation - Makszin Lilla
- 4. Microchip electrophoretic measurement, separation with Agilent 2100 Bioanalyzer - Makszin Lilla
- 5. Qualitative analysis of samples - Makszin Lilla
- 6. Quantitative analysis of samples, evaluation by help of 2100 Expert software - Makszin Lilla
- 7. Preparation of cell samples (bacteria, fungi, HeLa cells, etc.) - Makszin Lilla
- 8. Purification of cells - Makszin Lilla
- 9. Preparation of samples; labeling with fluorescent dyes - Makszin Lilla
- 10. Purification of labeled cells - Makszin Lilla
- 11. Microfluidic measurement, cell counting and evaluation with Agilent 2100 Bioanalyzer - Makszin Lilla
- 12. Final evaluation, mark - Makszin Lilla
Szemináriumok
A tananyag elsajátításához szükséges segédanyagok
Kötelező irodalom
Saját oktatási anyag
It is going to be available on the website and in Neptun.
Jegyzet
Ajánlott irodalom
Instrumental analysis lecture and practise; microchip electrophoresis
Ann Van Schepdael: Microchip Capillary Electrophoresis Protocols, Springer, 2015
G2938-90062 Agilent Protein 80 Reagent Kit GuideUser Manual 9/19/2006
G2938-90310 Agilent (2008) Agilent High Sensitivity Protein 250 Kit Guide
G2946-90004 Agilent 2100 Bioanalyzer 2100 Expert User's Guide User Manual 6/21/2005
A félév elfogadásának feltételei
Active participation and written test.
Félévközi ellenőrzések
-
Távolmaradás pótlásának lehetőségei
By personal consultation.
Vizsgakérdések
The required information for the test will be explained in the course.
Vizsgáztatók
Gyakorlatok, szemináriumok oktatói
- Makszin Lilla