Biochemistry - Practice

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2022-2023

Tematika

The course helps to evolve good practical skills. It gives an overview on the techniques that are used in the basic biochemical-, biomedical research, both in the industrial area as well as in clinical research. The main focus is on solving of different scientific problems and through that a solution orientated education.

Előadások

Gyakorlatok

• 1. Solutions
• 2. Solutions
• 3. Preparation of buffer solution
• 4. Preparation of buffer solution
• 5. Protein function
• 6. Basics of enzyme catalysis
• 7. Measuring the proteint contant of different solutions
• 8. Biuret reaction
• 9. Oral presentations
• 10. Oral presentations
• 11. Enzyme activity measurement
• 12. Inorganic phosphate demand of glycolysis
• 13. Oral presentations
• 14. Oral presentations
• 15. Molecular recognition
• 16. Affinity chromatography
• 17. Apoptosis, necrosis
• 18. Techniques to describe apoptotical processes or necrosis
• 19. PAGE, Western blot
• 20. PAGE, Western blot
• 21. Antibody-Based techniques in biochemistry
• 22. Antibody-Based techniques in biochemistry
• 23. Working with cells
• 24. Working with cells
• 25. Bioanalytical teachniques
• 26. Bioanalytical teachniques
• 27. Consultation
• 28. Exam

Szemináriumok

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Albert L. Lehninger - David L. Nelson - Michael M.Cox: Principles of Biochemistry, Worth Publichers, Inc.

Saját oktatási anyag

http://aok.pte.hu/hu/egyseg/oktatasianyagok/20

Jegyzet

http://aok.pte.hu/hu/egyseg/oktatasianyagok/20

Ajánlott irodalom

Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer: Biochemistry
Thomas M. Devlin: Textbook of Biochemistry. With Clinical Correlations, John Wiley & Sons, New York

A félév elfogadásának feltételei

Maximum of 15 % absence allowed

Félévközi ellenőrzések

Oral presentations should be kept by every student on the 5th or 7th week from an optional topic, in the from of a ppt presentation. The theme of the presentation should have a biochemical connection, and should be adjusted with the subject leader.

Távolmaradás pótlásának lehetőségei

There is no possibility to retake the practice.

Vizsgakérdések

A written exam that screens the basic methodological knowledge, and the capability of solving scientific problems.

Vizsgáztatók

Gyakorlatok, szemináriumok oktatói

• Dr. Juhász Kata
• Dr. Takátsy Anikó
• Nagyné Dr. Kiss Gyöngyi