Molecular Targets – Signal Transduction - practice

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2020-2021

Thematik

To demonstrate basic laboratory techniques in signal transduction.

Vorlesungen

Praktika

• 1. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 2. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 3. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 4. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 5. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 6. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 7. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 8. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 9. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 10. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 11. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 12. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 13. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 14. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 15. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 16. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 17. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 18. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 19. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 20. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 21. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
• 22. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
• 23. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
• 24. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
• 25. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
• 26. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
• 27. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
• 28. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Gomperts: Signal Transduction

Vom Institut veröffentlichter Lehrstoff

-

Skript

-

Empfohlene Literatur

Voraussetzung zum Absolvieren des Semesters

Maximum of 15 % absence allowed

Semesteranforderungen

-

Möglichkeiten zur Nachholung der Fehlzeiten

-

Prüfungsfragen

-

Prüfer

Praktika, Seminarleiter/innen

• Dr. Boldizsár Ferenc
• Dr. Prenek Lilla
• Kugyelka Réka