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Ausbildung

Molecular Development and Transdifferentation - practice

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2021-2022

Lehrbeauftragte/r

Semesterwochenstunden

Vorlesungen: 0

Praktika: 28

Seminare: 0

Insgesamt: 28

Fachangaben

  • Kode des Kurses: OBH-007-G
  • 2 kredit
  • Biotechnology MSc
  • Specialty in Medical Biotechnology modul
  • autumn
Voraussetzungen:

OBH-007-E parallel

Zahl der Kursteilnehmer für den Kurs:

min. 1 – max. 25

Thematik

Thje purpose of the subject is to provide laboratory training in basic embryological and stem-cell related analytical methods.

Vorlesungen

Praktika

  • 1. Alkaline phosphatase assay of JAR cells I.
  • 2. Alkaline phosphatase assay of JAR cells II.
  • 3. Invertebrate regeneration I
  • 4. Invertebrate regeneration II
  • 5. Histological analysis of invertebrate regeneration I
  • 6. Histological analysis of invertebrate regeneration II
  • 7. Isolation of eGFP bone marrow cells and hematopoietic reconstitution I
  • 8. Isolation of eGFP bone marrow cells and hematopoietic reconstitution II
  • 9. Visit to the IVF laboratory I
  • 10. Visit to the IVF laboratory II.
  • 11. Isolation of organs from mid-term mouse embryos I.
  • 12. Isolation of organs from mid-term mouse embryos II.
  • 13. In situ and ex vivo photoconversion of Kik photoprotein I.
  • 14. Isolation of organs from mid-term mouse embryos II.
  • 15. Phenotypic analysis of fetal organ-derived cultures: neural, liver, cardiac I.
  • 16. Phenotypic analysis of fetal organ-derived cultures: neural, liver, cardiac II.
  • 17. Analysis of hematopoietic stem cell engraftment in eGFP-transplanted mice I.
  • 18. Analysis of hematopoietic stem cell engraftment in eGFP-transplanted mice II.
  • 19. Isolation pf preimplantation mouse embryos and initiation of embryonic stem cell cultures I.
  • 20. Isolation pf preimplantation mouse embryos and initiation of embryonic stem cell cultures II.
  • 21. Passage of hatching embryos and expansion of eSC culture I.
  • 22. Passage of hatching embryos and expansion of eSC culture II.
  • 23. AP analysis of eSC cultures I.
  • 24. AP analysis of eSC cultures II.
  • 25. FACS sorting of tumor-inducing cells from Bc-DLFL.1 lymphoma cells I.
  • 26. FACS sorting of tumor-inducing cells from Bc-DLFL.1 lymphoma cells II.
  • 27. Consultation
  • 28. Consultation

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

n/a

Vom Institut veröffentlichter Lehrstoff

handouts and protocols provided at each practice

Skript

n/a

Empfohlene Literatur

n/a

Voraussetzung zum Absolvieren des Semesters

Maximum of 15 % absence allowed

Semesteranforderungen

n/a

Möglichkeiten zur Nachholung der Fehlzeiten

none

Prüfungsfragen

n/a

Prüfer

Praktika, Seminarleiter/innen

  • Dr. Balogh Péter
  • Dr. Engelmann Péter András
Rólunk