Applied Immunology - Practice and Seminar

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2024-2025

Thematik

Practices and seminars connetcted to Applied Immunology lectures.

Vorlesungen

Praktika

• 1.

  Brief review and recapitulation of the preparatory course material

  - Böröcz Katalin
• 2.

  Brief review and recapitulation of the preparatory course material

  - Böröcz Katalin
• 3.

  Reiteration the cell types present in the peripheral human blood through conducting Ficoll gradient centrifugation, buffy coat aspiration, cell counting, staining, and viability assessments

  - Böröcz Katalin
• 4.

  Reiteration the cell types present in the peripheral human blood through conducting Ficoll gradient centrifugation, buffy coat aspiration, cell counting, staining, and viability assessments

  - Böröcz Katalin
• 5.

  Utilizing an EDTA anti-coagulated blood sample for cell labeling in flow cytometry, revisit the subject of immune phenotyping and its significance

  - Böröcz Katalin
• 6.

  Utilizing an EDTA anti-coagulated blood sample for cell labeling in flow cytometry, revisit the subject of immune phenotyping and its significance

  - Böröcz Katalin
• 7.

  Microscope based examination of blood smears: recognition of various cell types, distinguishing between cell life and activation stages

  - Böröcz Katalin
• 8.

  Microscope based examination of blood smears: recognition of various cell types, distinguishing between cell life and activation stages

  - Böröcz Katalin
• 9.

  Monoclonal antibody (mAb) production, hybridoma technology. Immunization, preparation of antigen, ways of antigen delivery, serum antibody titer measurement.

  - Böröcz Katalin
• 10.

  Monoclonal antibody (mAb) production, hybridoma technology, Immunization, preparation of antigen, ways of antigen delivery, serum antibody titer measurement.

  - Böröcz Katalin
• 11.

  Monoclonal antibody (mAb) production, hybridoma technology, cell fusion

  - Böröcz Katalin
• 12.

  Monoclonal antibody (mAb) production, hybridoma technology, cell fusion

  - Böröcz Katalin
• 13.

  Monoclonal antibody (mAb) production, hybridoma technology, selection and cloning

  - Böröcz Katalin
• 14.

  Monoclonal antibody (mAb) production, hybridoma technology, selection and cloning

  - Böröcz Katalin
• 15.

  Hybridoma cell supernatant harvesting and purification (affinity chromatography)

  - Böröcz Katalin
• 16.

  Hybridoma cell supernatant testing - Preparing frozen tissue sections from mouse tissues for immunohistochemistry.

  - Böröcz Katalin
• 17.

  Hybridoma cell supernatant testing - Preparing frozen tissue sections from mouse tissues for immunohistochemistry.

  - Böröcz Katalin
• 18.

  Hybridoma cell supernatant testing – immunohistochemistry (IHC)

  - Böröcz Katalin
• 19.

  Hybridoma cell supernatant testing – immunohistochemistry (IHC)

  - Böröcz Katalin
• 20.

  Hybridoma cell supernatant testing –enzyme linked immunosorbent assay (ELISA)

  - Böröcz Katalin
• 21.

  Hybridoma cell supernatant testing –enzyme linked immunosorbent assay (ELISA)

  - Böröcz Katalin
• 22.

  Introduction to the autoimmue routine diagnostics laboratory - ELISA autoantibody diagnostics

  - Böröcz Katalin
• 23.

  Introduction to the autoimmue routine diagnostics laboratory ELISA autoantibody diagnostics

  - Böröcz Katalin
• 24.

  Introduction to the autoimmue routine diagnostics laboratory Automation

  - Böröcz Katalin
• 25.

  Introduction to the autoimmue routine diagnostics laboratory - Immunfluorescence

  - Böröcz Katalin
• 26.

  Introduction to the autoimmue routine diagnostics laboratory - Immunoblot

  - Böröcz Katalin

Seminare

• 27.

  Preparation for the exam – comprehensive seminar

  - Böröcz Katalin
• 28.

  Preparation for the exam – comprehensive seminar

  - Böröcz Katalin

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

none

Vom Institut veröffentlichter Lehrstoff

www.immbio.hu

Skript

none

Empfohlene Literatur

none

Voraussetzung zum Absolvieren des Semesters

Preparation of summary from the techniques and steps which were introduced during the practices.

Semesteranforderungen

Practical summaries must be submitted to the teacher.

Möglichkeiten zur Nachholung der Fehlzeiten

none

Prüfungsfragen

www.immbio.hu

Prüfer

Praktika, Seminarleiter/innen

• Böröcz Katalin