Applied Immunology - Practice and Seminar

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2024-2025

Tematika

Practices and seminars connetcted to Applied Immunology lectures.

Előadások

Gyakorlatok

• 1.

  Brief review and recapitulation of the preparatory course material

  - Böröcz Katalin
• 2.

  Brief review and recapitulation of the preparatory course material

  - Böröcz Katalin
• 3.

  Reiteration the cell types present in the peripheral human blood through conducting Ficoll gradient centrifugation, buffy coat aspiration, cell counting, staining, and viability assessments

  - Böröcz Katalin
• 4.

  Reiteration the cell types present in the peripheral human blood through conducting Ficoll gradient centrifugation, buffy coat aspiration, cell counting, staining, and viability assessments

  - Böröcz Katalin
• 5.

  Utilizing an EDTA anti-coagulated blood sample for cell labeling in flow cytometry, revisit the subject of immune phenotyping and its significance

  - Böröcz Katalin
• 6.

  Utilizing an EDTA anti-coagulated blood sample for cell labeling in flow cytometry, revisit the subject of immune phenotyping and its significance

  - Böröcz Katalin
• 7.

  Microscope based examination of blood smears: recognition of various cell types, distinguishing between cell life and activation stages

  - Böröcz Katalin
• 8.

  Microscope based examination of blood smears: recognition of various cell types, distinguishing between cell life and activation stages

  - Böröcz Katalin
• 9.

  Monoclonal antibody (mAb) production, hybridoma technology. Immunization, preparation of antigen, ways of antigen delivery, serum antibody titer measurement.

  - Böröcz Katalin
• 10.

  Monoclonal antibody (mAb) production, hybridoma technology, Immunization, preparation of antigen, ways of antigen delivery, serum antibody titer measurement.

  - Böröcz Katalin
• 11.

  Monoclonal antibody (mAb) production, hybridoma technology, cell fusion

  - Böröcz Katalin
• 12.

  Monoclonal antibody (mAb) production, hybridoma technology, cell fusion

  - Böröcz Katalin
• 13.

  Monoclonal antibody (mAb) production, hybridoma technology, selection and cloning

  - Böröcz Katalin
• 14.

  Monoclonal antibody (mAb) production, hybridoma technology, selection and cloning

  - Böröcz Katalin
• 15.

  Hybridoma cell supernatant harvesting and purification (affinity chromatography)

  - Böröcz Katalin
• 16.

  Hybridoma cell supernatant testing - Preparing frozen tissue sections from mouse tissues for immunohistochemistry.

  - Böröcz Katalin
• 17.

  Hybridoma cell supernatant testing - Preparing frozen tissue sections from mouse tissues for immunohistochemistry.

  - Böröcz Katalin
• 18.

  Hybridoma cell supernatant testing – immunohistochemistry (IHC)

  - Böröcz Katalin
• 19.

  Hybridoma cell supernatant testing – immunohistochemistry (IHC)

  - Böröcz Katalin
• 20.

  Hybridoma cell supernatant testing –enzyme linked immunosorbent assay (ELISA)

  - Böröcz Katalin
• 21.

  Hybridoma cell supernatant testing –enzyme linked immunosorbent assay (ELISA)

  - Böröcz Katalin
• 22.

  Introduction to the autoimmue routine diagnostics laboratory - ELISA autoantibody diagnostics

  - Böröcz Katalin
• 23.

  Introduction to the autoimmue routine diagnostics laboratory ELISA autoantibody diagnostics

  - Böröcz Katalin
• 24.

  Introduction to the autoimmue routine diagnostics laboratory Automation

  - Böröcz Katalin
• 25.

  Introduction to the autoimmue routine diagnostics laboratory - Immunfluorescence

  - Böröcz Katalin
• 26.

  Introduction to the autoimmue routine diagnostics laboratory - Immunoblot

  - Böröcz Katalin

Szemináriumok

• 27.

  Preparation for the exam – comprehensive seminar

  - Böröcz Katalin
• 28.

  Preparation for the exam – comprehensive seminar

  - Böröcz Katalin

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

none

Saját oktatási anyag

www.immbio.hu

Jegyzet

none

Ajánlott irodalom

none

A félév elfogadásának feltételei

Preparation of summary from the techniques and steps which were introduced during the practices.

Félévközi ellenőrzések

Practical summaries must be submitted to the teacher.

Távolmaradás pótlásának lehetőségei

none

Vizsgakérdések

www.immbio.hu

Vizsgáztatók

Gyakorlatok, szemináriumok oktatói

• Böröcz Katalin